Determination of Benzo (α) Pyrene Residue in Tea by HPLC with Fluorescence Detection
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Abstract:
A method for the determining of Benzo (α) pyrene residue in tea by HPLC with fluorescence detection (HPLC-RF) was developed. Samples were purified by SPE, and analyzed by HPLC-RF. Within the linear range of 0.2~50 g/L, the linear equation was Y=3.85×104X+7.47×103 (R=0.9991). The recovery tests of green and red teas ranged from 76.9% to 86.9%. The variation coefficients and detection limit were 2.0%~4.0% and 0.2 μg/kg (S/N=3), respectively. This method was suitable for the qualitative and quantitative analysis of Benzo (α) pyrene residues in tea, due to its simpleness, sensitivity, safety and high precision.
