The process of glucose production by two enzymes was studied. The processing conditions for glucose production were optimized and the best spurt temperature, liquefaction pH value, treat time at high temperature after rejection and total amylase amount were 108~110 ℃, 5.4-5.8, 5~7 min and 0.6 kg/t, respectively. It was also found that α-amylase with high thermal stability and new hydroheater should be adopted when the starch concentration was＞ 16 °Be. Besides, debranchins enzyme should be added during saccharification.
