Abstract:Pawpaw contained the flavonoids compound which can scavenge the activity oxygen free radicals in human body. In this paper, the crude flavonoids was extracted from pawpaw by ethanol and acetic ester, and then purified by AB-8 macroporous resins with the content of the flavonoids (rutin as standard) being of 4.8%. The structures of benzoyl ring (A-ring) and cinnamoyl ring (B-ring) were found in the extracted flavonoids bore and the spectrum characteristic of the extracts was similar to that of the rutine, which indicated that the extracts possessed the basic structure of flavonoids compounds, C6-C3-C6.