Abstract:The extraction, purification and antimicrobial activity of chlorogenic acid in tobacco leaves were researched. The optimal ultrasonic-assisted extraction conditions were as follows: 60.5% ethanol, 51.4 ℃ and 30.4 min. Under these conditions, the yield of crude tobacco chlorogenic acid was 1.6%. The crude chlorogenic acid was purified by NKA-9 resin. It was also found that the tobacco chlorogenic acid had inhibition activities on Escherichia coli, Staphylococcus aureus and Bacillus subtilis.
