Determination of 5-Hydroxymethylfurfural in Soy by HPLC
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Abstract:
A new method for the determination of 5-hydroxymethylfurfural by high performance liquid chromatography in soy was developed. After extracted by ethyl acetate, the samples were analyzed on a C18 column at 284 nm using a mixture of methanol and water (5:95, v/v) as the mobile phase . Within the linear range of 0.5~20.0 mg/L, the method was fast and accurate with the average recoveries rate and relative standard deviation of 82.4%~86.9% and 3.2%~5.1% , respectively. The minimum determination limit was 0.1 mg/L.
