Methods for Extracting GSH from Saccharomyces cerevisiae
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Abstract:
Four methods for extracting GSH from Saccharomyces cerevisiae were studied in this paper. It was found that GSH could be efficiently extracted by boiling water extraction, microwave assistant extraction, ultrasonic disruption and high-pressure homogenization. Under the optimized extraction conditions, the GSH concentrations by high-pressure homogenization, boiling water extraction, ultrasonic disruption and microwave assistant extraction were 3.975 mg/g, 3.830 mg/g, 3.823 mg/g and 3.593 mg/g, respectively. And the extraction rates by the above-mentioned four methods were 99.87%, 96.23%, and 96.12% and 90.28%, respectively. The “green” high-pressure homogenization was the most suitable one for industrial production among the examined extraction methods.
