Abstract:In this article ,we established a rapid method for detection of Lactobacillus in food using real-time PCR technique. General primers and specific probes were designed to detect the 16S rDNA sequence of Lactobacillus by TaqManTM real-time PCR assays using non-homologous strains for specific test. The results indicated this method was rapid, sensitive and with high specificity for the detection of Lactobacillus in food.
