Extraction, Isolation and Analysis of the Polysaccharides from Cordyceps Gunnii (Berk.) BerK.
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Abstract:
Two crude polysaccharides, CPS-50 and CPS-70, were extracted from the mycelium of Cordyceps gunnii (Berk.) Berk. using 30% ethanol. After remove of lipids (by ethyl acetate) and proteins (using sevage method), and precipitation (with 50% and 70% alcohol), the crude polysaccharides were further purified by DEAE-Sephadex A-25 column. The contents and purities of the obtained polysaccharides, named as CPS-1 and CPS-2, were determined using phenol-sulfuric acid method and UV spectrophotometric method, respectively. Their structures were analyzed by infrared spectrum (IR). In addition, thin layer chromatography analysis showed that the hydrolysis of the pure polysaccharides yielded two kinds of monosaccharides, glucose and/or mannose.
