Abstract:This experiment Pichia codon preference, a high sweetness synthetic gene Monellin, and the reorganization of production of yeast expression vector pPIC9M by electroporation can be a highly efficient expression of secretory activity sweet high sweetness Monellin reorganization Pichia GS115/pPIC9M. Monellin confirmed by PCR gene has been integrated into the yeast genome, SDS-PAGE and Western blot hybridization know immune expressed protein is the target protein final adoption of double-blind confirmed the presence of normal expression of activity.
