Determination of the Sialic Acid Content in Milk by HPLC
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Abstract:
Sialic acid in milk was released via acid hydrolysis, and then derivated with O-pheny-lenediamine •2HCl (10 mg/mL) at 80 ℃ for 40 min. The content of the derivated sialic acid was determinded on a Symmetry C18 column (250 mm×4.6 mm, 5 μm) using a UV detector at 230 nm. The mobile phase was a mixture of 1.0% tetraphdrofuran (containing 0.2% phosphoric acid) and acetonitrile (92:8) at a flow rate of 1.0 ml/min. The column temperatue was 35 ℃. The results indicated the linear rang of Sialic acid was 10~320 μg/mL and the average recovery was 95.7%. This method for the determination of the sialic acid content in milk had good reproducibility and high sensitivity.
