Abstract:In this study, glycolipids was extracted from heads of carp cyprinoids with chloroform and methanol. The best extraction conditions were determined by single factor test and as follows: the ratio of solid to liquid of 1:15, extracting temperature of 28 ℃ and stirring time of 24 h. The achieved crude glycolipid was then purified by column chromatography and the best developing solvents were the mixture of chloroform, methanol and water with their ratio being of 100:20:12:5. Besides, iodine gas was chosen as the best colour developing reagent.
