Determination of Chlorogenic Acid in Sunflower Kernel by Thin Layer Chromatography-spectrophotometry
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Abstract:
A new method for determination of chlorogenic acid in sunflower kernel by thin layer chromatography-spectrophotometry was established. The chlorogenic acid in the sample was separated by thin layer chromatography. Analytical conditions of the chlorogenic acid were optimized and the effects of some key factors on the determination were discussed. Within the range of chlorogenic acid concentration of 0.20~1 μg/mL, the linear regression equation was A=0.0451c+0.897, with the linear correlation coefficient of 0.9995.
