Abstract:The selection and enumberation of Lactococcus Q26 was studied. The results showed that the sucrose, N-sources, the ratio of Peptone and Beef extract and Yeast extract, Yam extract, CaCO3, Vc, and Tomato extract of culture medium is 0.5%, 2.5%, 2:2:1(m/m/m), 5%, 0.3%, 0.3% and 2.5%, respectively. The highest number of Lactococcus can reach 2.36×109 /mL.