Abstract:The preparation of anticoagulant cellulose sulfate was investigated by five factors quadri-rotation orthogonal experiments and statistical optimization analysis. The results showed that the optimum preparation temperature of esterifying agent, esterifying agent dosage, reaction temperature, reaction time and pH value were 20.0 ℃, 6.8 mol/mol AGU, 12 ℃, 3.5 h and 8.0, respectively, under which, the cellulose sulfate had the highest effect on the prolongation of the RT, APTT and TT, meaning that the best anticoagulant activity of cellulose sulfate was achieved.
