Magnetic Affinity Support for Trypsin Separation and Purification
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Abstract:
Ultrafine high magnetic affinity support was prepared by coating nanometer barium ferrite with chitosan, cross-linking with epichlorohydrin, and then activating with carbodiimide. The analysis result of the dynamic laser scatters indicated that the average diameter of the magnetic particles was 401 nm. The magnetic measurement showed that the ultrafine magnetic particles were high magnetic. At first the aprotinin was considered to be affinity ligand and covalently bound onto the magnetic particles via carbodiimide activation, and then these particles were used for the affinity purification of trypsin. The proposed method was successfully applied to the affinity purification of trypsin with the purified conditions of trypsin affinity under discussing.
