Abstract:The absorption characteristic of α-arbutin produced by Aspergillus oryzae and its separation and purification were studied. Among the examined absorbent resins, the S-8 resin was found to be the best one. The conditions of separation and purification of α-arbutin using S-8 resin were optimized and the best pH value of samples, absorption time, loading amount of the samples and the flow rate of water eluent were 1, 1 h, 0.2 mg/g Resin, and 3 BV/h, respectively, under which the purity of α-arbutin reached 99%.