Preliminary Research on the Measurement of Fatty Acid Composition of the Vegetable Oil by Capillary Column Chromatography
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Abstract:
Pure methyl esters were prepared using common oil plant seeds and the fatty acid composition of the achieved oil was measured by capillary column chromatography. The kinds and dosage of reagents for fast preparation of the methyl esters were investigated and the chromatographic conditions using two kinds of capillary columns were optimized. Under the determined conditions, the fatty acid contents in the oil were in accord with the national standards. Compared the standards values with ECL values, the method was steady and credible, because the difference of comparison was little. This simple, fast, accurate method was suitable for determining fatty acid composition of samples in large quantities.
