Abstract:The influences of cultivation conditions on the cordycepin production via submerged cultivation of Cordyceps militaris in the shake flask, were investigated by single factor and orthogonal experiments. The results indicated that the optimal temperature, shaking speed and initial pH value for cordycepin production were 25 ℃, 150 r/min and 5.0, respectively, under which 16.20 g/L of biomass and 264.00 mg/L of cordycepin were achieved.