Abstract:Objective: To research the differential expression of liver tissue proteins in the Alloxan-Induced hepatic injury in mice by using the simpler method to extract the proteins of liver tissue in mice and provide the theoretical study of the mechanism of alloxan-induced hepatic injury. Methods: The mice were divided into two groups. In the model group, the mice were injected with alloxan and in the control group they were injected with 0.9% brine. Then the liver tissues of the killed mice in the two groups were investigated on aspect and histology. Proteins in the liver tissue were deposited into different grades and the attained proteins were separated by SDS-PAGE grads electrophoresis with colloidal staining method. The differential expressions of proteins in the model group and the control group were analyzed and the peptide mass fingerprints were detected with MALDI-TOF-MS. The corresponding proteins were retrieved in Databank. Results: The differential depressions of proteins were found both in the water-soluble proteins and non-water-soluble proteins in the two groups. One of the differential proteins in water-solubility-protein extracted by 50% acetone was choose for further analysis and was found to be an unnamed protein.
