Initial Screening of High-yield Yeast Strain and Optimization of Nutritional Conditions for Glutathione(GSH)
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Abstract:
Comparing the yield of glutathione from 14 different species of yeast strains kept in the Key Lab of Hubei Industrial Microbiology, it was found that the highest glutathione yield was 127.785 mg/L achieved by Saccharomycodes ludwigii log. The nutritional conditions were further investigated. Results showed that the optimum carbon source was glucose. Nitrate could inhibit the growth of yeasts, and organic nitrogen sources could benefit the growth. For the growth of yeast cell and the metabolism in fermentation, KH2PO4 was selected as one of the basic ingredients of culture medium. The basic medium ingredients were also optimized with orthogonal test. The yield of GSH by Saccharomycodes ludwigii log could be greatly improved and up to148.547 mg/L.
