Abstract:An indirect competitive enzyme-linked immunosorbent assay (ELISA) procedure was established to detect ractopamine employing a polyclonal antibody generated from RAC-linker-BSA and a coating antigen synthesized by method of mixed acid anhydride. The antibody against ractopamine showed high sensitivity with an IC50 of 0.9 ng/mL and the detection limit (IC15) was 0.1 ng/mL, which was lower than the data reported in literatures. The antibody with high specificity showed no cross-reactivity with clenbuterol, salbutamol, isoproterenol, terbutaline and isoxsuprine, while had the cross-reactivity of 7.5% with dobutamine. In addition, it was stable enough to be stored at 4 ℃ for half a year. This study laid a well foundation for the control of ractopamine by ELISA method.
