The enzymolysis conditions of cellulose enzyme for extraction of Capsaicinoids, including the initial pH value of lytic enzyme solution, reaction time and temperature, were optimized here by orthogonal test. The optimized conditions were that the initial pH value was 5.4, temperature was 55 ℃, reaction time was 4 h and the quantities of enzyme was 2.5 mg per 1 g capsicum, the yield of capsaicin was 7.65 mg/g, which is improved by 33% compared to that by traditional method.
