Abstract:A comparison of the growth of on TPY, PTYG and modified MRS medium was made and PTYG medium was shown to be the best one. Results also indicated that both the hydrolyzed whey protein and lactose could affect the growth of B. bifidum in PTYG medium and the most suitable additive amounts of hydrolyzed whey protein and lactose were 10% (mL/mL) and 1.5% (g/g), respectively.