Study on Isolation, Purification and the Free-Radical Scavenging Effect of Proanthocyanidins from Grape Seed (2)
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Abstract:
The content of proanthocyanidins monomer extracted by ethyl acetate or ether was confirmed by thin layer chromatography scan (TLCS), and the results were compared with those by vanillin-HCI method. The effects of pH value, temperature, light, treatment with Vc and sodium hydrogen sulfite on the stability of proanthocyanidin in grape seeds were also investigated. Then the microcapsule of proanthocyanidins was made using β-cyclodextrin as coating material. The results showed that the contents of monomer in ethyl acetate and ether extracts by TLCS were 29.02% and 59.98%, respectively, which were in accordance with those by vanillin-HCI method. Proanthocyanidins was stable without light at low pH value (3~5) and room temperature. 0.6% Vc or 0.75% sodium hydrogen sulfite showed very strong protective effect on proanthocyanidins. When 10 g β-cyclodextrin and 0.189 g proanthocyanidins were used, the output and embedding efficiency of Proanthocyanidins microcapsule could reach 80.4% and 20.5%.