Detection of BT Transgenic Rice Gene by Polymerase Chain Reaction
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Abstract:
Polymerase Chain Reaction (PCR), an important method for the identification of genomic modified foods (GMF), was developed for detection of the BT transgenic rice gene. Using this method, 35S-promoter gene, NOS-terminator gene, and cry1Ac gene that commonly existed in transgenic rice were detected. Moreover, new methods based on PCR techniques were established for distinguishing Bt Transgenic Rice from the counterpart of non-GM products and the detection system and for correlating the marked genes and transgenic. The method was easy and fast, and the corresponding results were in accord with the standard or the reported data.
