Abstract:The protein in soybean residue was hydrolyzed by various proteinase and two enzymes, papain and flaourzyme, were chosen for this purpose. The most suitable ratio of papain to substrate, ratio of flavourzyme to substrate, the reaction rime by flavourzyme, whole time of the hydrolysis and the substrate concentration were 600 U/g; 1200 U/g; 14.5 h, 16 h and 2%, respectively. After hydrolysis, the recovery of the total nitrogen reached 55.73%.