Extraction and Isolation of Glycosaminoglycan from Chlamys Nobilis and Study on its Anti-tumour Effect
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Abstract:
Glycosaminoglycan (GAG) from the whole viscera of Chlamys nobilis was achieved via enzymatic hydrolysis by papain and neutral proteinase and ethanol precipitation. Its anti-tumor activity was also examined by MTT colormetric assay. Results showed that the yield of crude product reached 1.26%, and the content of total sugar and GAG were 41.4% and 26.9%, respectively. Besides, it showed significant anti-tumor activity towards Hela cell (p<0.05).
