In this project Xy-1 and Xy-5 were successfully selected form the nature with high xylanase-producing activity. The xylan was prepared using corncob as raw material and the conditions of enzymatic hydrolysis and extraction of xylan were also discussed. The results showed that the best reaction temperature, pH value, and reaction time for the enzymatic hydrolysis of xylan were 50 ℃, 5.0 and 48 h, respectively. For its extraction, the most suitable NaOH content, temperature and extraction time were 10%, 85 ℃ and 4h, respectively.
