Antioxidant activity of spinach extracts was studied in this paper. Antioxidants from spinach were extracted by different polar organic solvent and the total phenolic compounds, total flavones and DPPH radical scavenging activity were all determined .It was found that the optimal ethanol concentration was 95%, with which the extracting ratio, content of total phenolic compounds and content of total flavones reached 4.96%, 39.60 µg/mg and 17.53 µg/mg, respectively. Besides, DPPH radical scavenging capacity was 62.09 % in 0.5 mg/mL extracts.
