Determination of Synthetic Colors in Coating Tablet of a Health Food by HPLC
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Abstract:
High performance liquid chromatography (HPLC) was used to determinate the content of artificial synthetic colorsin coating tablet of a healthful food, including tartrazine, indigo carmine and allure red. After purified by polyamide, the samples were assayed by HPLC on a Lichrospher C18 column using a Dual Absorbance Detector. The mobile phase was a mixture of methanol and ammonium acetate (pH 4.0, 0.02 mol/l), with a liner gradient of: methanol from 20% to 35% in 5 min, continuing to increase methanol content in the mobile phase from 35% to 98% in 7 min, and then maintaining for 6 min in 98%. The flow rate was 1 ml/min and solo-wavelength was set at UV 254 nm. The optimal multi-wavelength for Tartrazine, Indigo Carmine and Allure red were 428 nm, 610 nm and 500 nm, respectively. Results also showed that the linearity range, recovery range, relative standard deviation and the detection limit were 0~100 μg/ml, 91.3%~103.1%, 2.11%~5.63%, and 2 ng ~11 ng, respectively.
