Determination of Fructose Content in Malus micromalus Makino by UV-Spectrometry

Abstract:

Under acidic conditions, fructose can be dehydrated to 5-hydroxymethylfurfural. The UV-spectrometry analysis showed that the dehydration product had maximum absorption at 285nm. The linear range with this method is 0~34g/ml and the regress formula is: y=0.0322x+0.0128 (R=0.9987). The analysis using this method also showed that the recovery ratio could reach 99%.