Study on Optimization of Extraction Technology of Saponin from Momordica charantia L.
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Abstract:
In this paper, the extraction technology of saponin in momordica chrantia L. and methods for measuring saponin were studied. The quantitative analysis of saponin extracted from momordica charantia L was made by spectrophotometry using the Ginsenoside Rg1 standard as reference. Several influential factors in the extraction of spoin from momordica chrantia L, including solid-liquid ratio, temperature, mass fraction of alcohol, time, were investigated and optimized by means of orthogonal rotational combination design. The results indicated that the effects of factors on the extraction were in the order of extraction time>solid-liquid ratio>mass fraction of alcohol >temperature. The frequency analysis showed that the optimal solid-liquid ratio, temperature, mass fraction of alcohol and extraction time were 10.190~11.016, 68.80~72.33℃, 71.32~74.32% and 13.484~19.396h, respectively, under which the extraction rate of saponin was above 2.18%. Additionally, the mathematic model was well established to estimate the extraction rate of saponin from momordica charantia L. within the investigated range. From a practical viewpoint, Solid-liquid ratio, temperature, mass fraction of alcohol and extraction time was considered to be 1:10, 70℃, 70% and 12h, respectively, which were close to both the practical production and the theoretical values.
