The Preparation of Immobilized Lipase by Embedded in Sodium Alginate
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Abstract:
The conditions of the immobilized lipase using sodium alginate as carrier were studied. First, sodium alginate solution (dissolved with hot distilled water) and 500 mg lipase solution (dissolved with buffer solution (pH=6)) were mixed and the obtained mixture was dropped into 1% aseptic CaCl2 solution using an injector. After immobilized for 1h with shaking, filtration, washing and drying, the round immobilized lipase was finally obtained with good thermal stability. The residue activities of the immobilized lipase and free lipase after incubated at 60℃ for 1.5h were 70% and 18%, respectively. Moreover, the immobilized lipase had good operational stability, which could be repeatedly used for 10 times and the relative activity of the lipase was as high as 95%.
