A mutant Bacillus subtilis Tc142 producing uridine was obtained by DES and subsequent UV mutagenesis using wild type Bacillus subtilis TJ174 as the parent strain. The mutant Tc142 has been marked with 2 TUr、6 AUr and UP, using which, uridine content in the fermentive liquor at 36℃ after 72 h reached 3.34g/L.