基于功能化边缘改性石墨烯表面修饰的葡萄糖电化学生物传感器的构筑

Construction of an Electrochemical Biosensor for Glucose Based on Functionalized Edge-modified Graphene Surface Modification

摘要:

为了实现对葡萄糖的快速、高灵敏检测，该研究采用二维碳基纳米材料功能化边缘改性石墨烯（FG）作为导电载体，葡萄糖氧化酶（GOx）作为生物识别分子，构建了检测葡萄糖的高性能电化学生物传感器（GCE/FG/GOx/CS）。实验采用循环伏安法（CV）和电流-时间曲线法（i-t），探讨了GCE/FG/GOx/CS电极的电化学传感性能。结果表明，该传感电极具有良好的电传感性能，对葡萄糖具有较高的检测灵敏度（46.85 μA•mmol•L-1•cm-2）和较宽的线性检测范围（0.05~1.60 mmol•L-1），检测限低至2.37 μmol•L-1。此外，该电极还具有良好的选择性、重现性（n=7，相对标准偏差RSD=2.05%）、循环稳定性和贮存稳定性（一周后响应电流保持率为97.09%）。用于实际饮料样品中葡萄糖含量的测定时，其加标回收率为98.5%~104.4%。该方法操作简便、选择性好、灵敏度高，为实现食品中葡萄糖含量的快速检测提供了理论依据。

Abstract:

To achieve rapid and highly sensitive detection of glucose, two-dimensional carbon-based nanomaterials functionalized edge-modified graphene (FG) were used as a conductive carrier and glucose oxidase (GOx) as a biorecognition molecule to construct a high-performance electrochemical biosensor for glucose detection (GCE/FG/GOx/CS). The electrochemical sensing performance of GCE/FG/GOx/CS electrodes was investigated using cyclic voltammetry and time-current methods. The sensing electrodes showed good electrosensing performance, high sensitivity (46.85 μA•mmol•L-1cm-2), and a wide linear detection range (0.05~1.60 mmol•L-1) for glucose, with a detection limit as low as 2.37 μmol•L-1. In addition, the electrode showed good selectivity, reproducibility (n=7, relative standard deviation RSD=2.05%), cycling stability, and storage stability (97.09% retention of response current after one week). The recoveries were 98.5%~104.4% for glucose determination in actual beverage samples. The method is simple, highly selective, and sensitive, providing a theoretical basis for the rapid detection of glucose content in food.