Abstract:To investigate the effects of D-allulose on dough properties and bread quality, dough and bread samples were prepared by partially replacing sucrose with D-allulose at concentrations of 0%, 4%, 8%, 12%, and 16% (wt.%). Variations in dough flour properties, fermentation behavior, textural attributes, microstructure, water distribution, and sensory quality of bread were systematically examined. Results demonstrated that as the concentration of D-allulose increased, the water absorption capacity of dough rose, the formation time gradually shortened, the weakening degree progressively increased, and the stability time exhibited a trend of initially increasing and subsequently decreasing. A D-allulose concentration of 16% was detrimental to dough formation and stability. Moderate incorporation of D-allulose (4%~8%) effectively enhanced the gluten network, thereby preventing gas loss during fermentation. In contrast, high concentrations of d-allulose (12%~16%) inhibited fermentation activity and reduced gas production rates. The hardness, adhesiveness, and chewiness of dough increased with higher D-allulose levels, whereas elasticity decreased and cohesiveness remained relatively unchanged. Notably, dough in the 16% group exhibited a significantly greater hardness and poorer elasticity. D-Allulose contributed to a more continuous and compact gluten network structure, improving the water-holding capacity of dough. Sensory evaluation revealed that an addition of 8% D-allulose enhanced the baking color, flavor, and internal texture of bread. Conversely, the sensory quality of bread in the 16% group was markedly diminished. This study provides valuable insights into the application of D-allulose in bread formulation.