构树穗多糖的抗氧化活性及对AAPH诱导的人红细胞氧化损伤保护作用

Antioxidant Activity of Polysaccharide from Broussonetia papyrifera panicle and Its Protective Effect Against AAPH-induced Oxidative Damage in Human Red Blood Cells

摘要:

该研究探讨了构树穗多糖的抗氧化活性及对AAPH诱导的人红细胞氧化损伤保护作用。该研究以构树穗为原料，采用热水浸提法设计单因素试验，用响应面法优化得到构树穗粗多糖提取工艺。以ABTS+•、DPPH•、•OH清除率，Fe3+还原能力和对AAPH诱导的人红细胞氧化损伤保护作用为考察指标，研究构树穗多糖的体外抗氧化活性和抑制人红细胞溶血性能。实验表明，最佳的提取工艺条件为：料液比1:32.33（g/mL），提取温度89.89 ℃，提取时间116.53 min，此时构树穗粗多糖得率23.67%；在抗氧化实验中，构树穗多糖对ABTS+•、DPPH•、•OH清除率分别达到了76.92%、57.81%、80.76%。构树穗多糖对Fe3+的还原能力在质量浓度8 mg/mL，波长700 nm条件下的吸光度为1.66；红细胞溶血实验表明，构树穗多糖可以有效地抑制AAPH处理的红细胞内ROS和MDA的产生，并降低GSH转化为GSSG的转化率，同时调节CAT、GSH-Px和SOD的酶活性恢复到正常红细胞的水平。研究证明了构树穗多糖具有良好的抗氧化能力，可以保护红细胞免受AAPH造成的氧化损伤。本研究旨在为研究构树穗多糖具生物活性的物质基础提供科学依据，对构树穗保健食品研发具有重要意义。

Abstract:

In this study, the antioxidant activity and protective effect against AAPH-induced oxidative damage in human erythrocytes of the polysaccharides from Broussonetia papyrifera panicle were studied. Broussonetia papyrifera panicle was taken as the raw material, single factor experiments were designed for a hot water extraction method and the extraction process of Broussonetia papyrifera panicle crude polysaccharide was optimized by the response surface method. The evaluation indicators included the scavenging rate of ABTS+∙, DPPH∙ and ∙OH, the reducing ability of Fe3+, and the protective effect against the AAPH-induced human red blood cell oxidative damage, to study the in vitro antioxidant activity and hemolysis inhibition of the polysaccharides. The results showed that the optimal conditions were: solid-to-liquid ratio 1:32.33 (g/mL), extraction temperature 89.89 ℃, extraction time 116.53 min, under which the yield of crude polysaccharide was 23.67%. In the antioxidant experiments, the scavenging rates of ABTS+∙, DPPH∙ and ∙OH of the polysaccharide were 76.92%, 57.81% and 80.76%, respectively. The absorbance of the polysaccharide corresponding to the reducing ability against Fe3+ was 1.66 at a polysaccharide mass concentration of 8 mg/mL and the wavelength of 700 nm. Erythrocyte hemolysis experiments showed that the polysaccharide could effectively inhibit the production of ROS and MDA in the erythrocytes treated by AAPH, reduce the conversion rate of GSH to GSSG, and regulate the enzyme activities of CAT, GSH-PX and SOD to restore normal red blood cell levels. The study confirmed that the polysaccharide from Broussonetia papyrifera panicle possessed good antioxidant capacity and can protect erythrocytes from oxidative damage caused by AAPH. This research aimed to provide a scientific foundation for the study on the bioactive material basis of Broussonetia papyrifera panicle polysaccharides, which is of great significance for the research and development of health food products derived from Broussonetia papyrifera panicle.