高溶氧水对急性缺氧小鼠的保护作用

Protective Effect of Highly Oxygenated Water in Acutely Hypoxic Mice

摘要:

为了研究高溶氧水对急性缺氧小鼠脑组织的保护作用，该研究将80只SPF级雌性昆明种小鼠随机分为对照组和高溶氧水组，处理30 d后随机分为4组，每组10只，其中三组用于测定常压缺氧实验、亚硝酸钠中毒实验和急性脑缺氧实验的存活时间；剩余20只常压缺氧死亡后迅速摘取脑组织，检测其HIF-1α、VEGF的含量，HIF-1α、VEGF及AMPK的蛋白相对表达量和mRNA水平。与对照组相比，高溶氧水组三种缺氧实验的存活时间均被延长，延长率分别为6.79%、28.02%、10.83%，该组HIF-1α（P<0.05）和VEGF（P<0.01）含量均明显减少，HIF-1α、VEGF蛋白表达量出现下调（P<0.05），HIF-1α mRNA水平增加（P<0.01）。研究结果表明，高溶氧水可以在急性缺氧时促进细胞组织通过消耗HIF-1α来快速适应缺氧，同时调节HIF-1α的转录过程，多向发挥耐缺氧作用。结果表明短期饮用高溶氧水对急性缺氧小鼠的脑组织具有保护作用。

Abstract:

To investigate the protective effect of highly oxygenated water on the brain tissue of acutely hypoxic mice, a total of 80 SPF female Kunming mice were randomly divided into a control group and a highly oxygenated water group. After 30 days of treatment, the mice were further divided into four groups of 10 mice each for subsequent analysis. Three groups were used to determine the survival time in the atmospheric hypoxia experiment, sodium nitrite poisoning experiment, and acute cerebral hypoxia experiment. Additionally, brain tissue samples from the remaining 20 mice were immediately removed following death as a result of atmospheric pressure hypoxia, to detect the levels of HIF-1α and VEGF, as well as to assess the protein expression and mRNA levels of HIF-1α, VEGF, and AMPK. The results showed that, compared to the control group, the highly oxygenated water group exhibited extended survival times by 6.79%, 28.02%, and 10.83% in the three hypoxia experiments, respectively. Furthermore, the HIF-1α (P<0.05) and VEGF (P<0.01) levels were significantly reduced, the protein expressions of HIF-1α and VEGF were downregulated (P<0.05), and the mRNA level of HIF-1α was significantly increased (P<0.01). These results suggest that the intake of highly oxygenated water can promote the rapid adaptation of cells and tissues to acute hypoxia by depleting HIF-1α levels, regulating its transcription, and exerting a multi-directional hypoxia tolerance effect. In summary, short-term consumption of highly oxygenated water shows a protective effect on the brain tissue of acutely hypoxic mice.