元宝枫籽油纳米结构脂质载体对秀丽线虫衰老的改善作用

Antiaging Effects of Acer truncatum Seed Oil-based Nanostructured Lipid Carriers on Caenorhabditis elegan

摘要:

该文对熔融乳化-超声法制备得到的元宝枫籽油纳米结构脂质载体（ASO-NLC）性状进行表征，并考察其对秀丽隐杆线虫寿命、生育能力、运动能力、抗氧化能力、热/氧化应激及细胞凋亡的影响。结果表明，ASO-NLC粒径为244.40 nm，PDI为0.17，zeta电位为-30.07 mV，离心稳定性好，是一种稳定性良好的口服制剂。与空白组比较，ASO-NLC可使线虫最长寿命延长50.21%以上，平均寿命延长12.00%~36.00%，而且寿命的延长不会导致线虫生殖能力下降；线虫培养10 d后，ASO-NLC能显著提高线虫的蠕动能力，到15 d时，作用更为明显。ASO-NLC可使谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）等抗氧化酶活性分别提高21.58%~48.81%、22.42%~58.58%和16.11%~75.88%；丙二醛（MDA）含量降低6.81%~34.89%。急性氧化应激下，ASO-NLC组线虫最长存活时间延长了1.50倍。吖啶橙染色结果显示，ASO-NLC组相对荧光强度比空白组减弱了13.71%~17.00%。综上所述，ASO-NLC可通过延长线虫寿命，提升其运动能力，提高线虫抗应激损伤的能力、体内抗氧化酶活性及减少细胞凋亡来延缓线虫衰老。该研究为元宝枫籽油功能性多元化产品开发提供了理论基础。

Abstract:

The properties of Acer truncatum seed oil-based nanostructured lipid carriers (ASO-NLC) prepared using the melt emulsification-ultrasonication method were characterized, and their effects on the longevity, fertility, exercise capacity, antioxidant capacity, heat/oxidative stress, and apoptosis of Caenorhabditis elegans were evaluated. ASO-NLC exhibited a particle size of 244.40 nm, a polydispersity index of 0.17, a zeta potential of -30.07 mV, and good centrifugal stability, that it is a stable oral formulation. Comparisons between the treatment groups and the control group (NC) revealed that ASONLC could extend the longest lifespan by more than 50.21% and the average lifespan by 12%~36%. Moreover, the extension of life span did not reduce the fertility of C. elegans. ASO-NLC also significantly improved the motility of C. elegans after 10 days of culture, with more obvious enhancement observed on day 15. Additionally, ASO-NLC increased the activities of glutathione peroxidase, catalase, and superoxide dismutase by 21.58%~48.81%, 22.42%~58.58%, and 16.11%~75.88%, respectively, while reducing the malondialdehyde content by 6.81%~34.89%. Under acute oxidative stress, the longest survival time of C. elegans in the ASO-NLC group was 1.50-fold longer than that in the NC group. The acridine orange staining results demonstrated that the relative fluorescence intensity of the ASO-NLC group was 13.71%~17.00%, which was significantly lower than that of the NC group (P<0.01). In conclusion, ASO-NLC can increase the lifespan, exercise capacity, and stress resistance of C. elegans; enhance the in vivo activities of antioxidant enzymes; and reduce apoptosis, enabling ASO-NLC to slow the aging of C. elegans. This study provides a theoretical basis for the development of functional products of A. truncatum seed oil.