不同花色睡莲品种黄酮和酚类物质含量及抗氧化能力分析

Analysis of flavonoid and phenolic substance contents and antioxidant capacities in different color varieties of water lilies

摘要:

本文对10个睡莲品种花瓣的花色、总黄酮与总酚含量、含水量、pH、提取物抗氧化活性等进行综合分析,其中黄金国睡莲花瓣中总黄酮含量最高,为20.40 mg/g,紫乔伊睡莲花瓣中总酚含量最高,为31.22 mg/g；而银河系睡莲花瓣中两者含量最少,分别为4.85 mg/g和5.21 mg/g。总黄酮含量与亮度L*(R=0.652)和色相b*(R=0.645)表现出显著正相关(P<0.05),与色相a*(R=-0.733)表现出显著负相关性(P<0.05)。10种睡莲花瓣所含有的黄酮与pH、含水量均无明显的相关性。在DPPH法中,红色闪耀睡莲花瓣黄酮提取液清除能力最强,EC50为2.51±0.19 μg/mL；其次是多丽丝,EC50为4.51±0.32 μg/mL,清除能力强于VC,其余品种花瓣黄酮提取液清除能力都弱于VC；ABTS法中,紫贝壳睡莲花瓣黄酮提取液清除能力最强,EC50为24.31±0.52 μg/mL,并且在铁离子还原/抗氧化能力(FRAP)法中也表现较好,但各品种睡莲清除能力均弱于VC。综合分析,不同品种睡莲花瓣pH、总黄酮、总酚含量及抗氧化活性差别较大,大多数睡莲花瓣均含有较高的黄酮含量,表明其抗氧化活性较好,因此具有作为良好的天然抗氧化剂进一步开发潜力。

Abstract:

Water lily plants are rich in bioactive substances, and the tissues contain flavonoids and other components, which can be widely used in the food industry and the field of medicine and cosmetics. In this paper, 10 kinds of water lily petals were comprehensively analyzed for flower color, total flavonoids and total phenols content, water content, pH, and antioxidant activity of the extracts, etc. The results showed that the petals of the Nymphaea ‘Eldorado’ contained the most total flavonoids, 20.40 mg/g; the petals of the Nymphaea ‘Purple Joy’ contained the most total phenols, 31.22 mg/g; and the least in the Nymphaea ‘Galaxy’, 4.85 mg/g and 5.21 mg/g, respectively. Total flavonoids content showed significant positive correlation with brightness L* (R=0.652) and color phase b* (R=0.645) (P<0.05), and significant negative correlation with color phase a* (R=-0.733) (P<0.05). Flavonoids contained in the petals of the 10 water lily species did not have any significant correlation with pH and water content. In the DPPH method, the Nymphaea ‘Red Flare’ petals flavonoid extract showed the best scavenging ability, with an EC50 of (2.51±0.19) μg/mL; the Nymphaea ‘Doris Holt’ showed better performance, with an EC50 of (4.51±0.32) μg/mL, and the scavenging ability was stronger than that of VC, while the scavenging ability of the remaining species" petal flavonoid extracts was weaker than that of VC. In the ABTS method, the scavenging ability of the Nymphaea ‘Zi Beike’ petals flavonoid extract showed the best performance, with EC50 of (24.31±0.52) μg/mL, and it also showed especially outstanding performance in the FRAP method, but the scavenging ability of each variety of waterlily was weaker than that of VC. In the comprehensive analysis, the pH, total flavonoids, total phenols content and antioxidant activity of the petals of different varieties of waterlily varied considerably. Most of the waterlily petal contain high flavonoid content, indicating good antioxidant activity, and thus have the potential for further development as a good natural antioxidant.