基于非靶向代谢组学揭示山葵不同部位差异代谢物

Untargeted Metabolomics Analysis of Differential Metabolites in Different Parts of Wasabia japonica Matsum

摘要:

为了系统揭示山葵须根、根茎、叶片三个部位的代谢物组成差异及其调控机制，该研究采用非靶向代谢组学技术，对山葵不同部位的代谢物进行鉴定，并运用主成分分析和正交偏最小二乘判别分析对代谢物差异进行分析，进一步开展了KEGG通路富集分析。共鉴定出2883种代谢物，筛选出1741个显著差异代谢物，在根茎vs须根、叶片vs须根、根茎vs叶片比较组中，分别筛选出883、1213和1108个差异代谢物，这些差异代谢物主要涉及黄酮类、萜类、香豆素、生物碱等功能性成分。主要代谢途径包括甜菜碱生物合成、黄酮类化合物生物合成、色氨酸代谢和亚油酸代谢等。该研究表明，山葵不同部位代谢物的显著差异及潜在的生物功能，为开发新型功能性食品和天然食品添加剂提供了重要的理论依据，同时也为深入研究山葵的代谢调控机制奠定了基础。

Abstract:

To systematically reveal the differences in metabolite composition and regulatory mechanisms among three parts of Wasabia japonica Matsum: fibrous roots, rhizomes, and leaves, untargeted metabolomics technology was employed. The metabolites in these parts were identified and analyzed for differences using principal component analysis and orthogonal partial least squares-discriminant analysis, followed by KEGG pathway enrichment analysis. A total of 2883 metabolites were identified in the fibrous roots, rhizomes, and leaves of Wasabia japonica Matsum, and 1741 significantly different metabolites were screened out. In the comparative groups of rhizome vs fibrous roots, leaf vs fibrous roots, and rhizome vs leaf, 883, 1213, and 1108 differential metabolites were identified, respectively. These differential metabolites were found to mainly consist of flavonoids, terpenoids, coumarins, alkaloids, and other functional components. The major metabolic pathways of the differential metabolites were determined to be betaine biosynthesis, flavonoid biosynthesis, tryptophan metabolism, and linoleic acid metabolism. The significant differences in metabolites among different parts of Wasabia japonica Matsum were elucidated, along with their potential biological functions, providing an important theoretical basis for the development of novel functional foods and natural food additives, and also laying the foundation for further in-depth research on the metabolic regulatory mechanisms of Wasabia japonica Matsum.