Abstract:Peanut meal is a high-quality protein source, but is prone to aflatoxin contamination. Therefore, aflatoxin detoxification in peanut meal is of significant importance for ensuring feed safety. In this study, four strains with the potential for aflatoxin B1 (AFB1) detoxification were initially screened from traditional sauerkraut using coumarin medium. A comparative study of the four strains showed that Lactobacillus paracasei YS-816 exhibited the highest detoxification rate of 70.05% for AFB1; and its AFB1 detoxification rate in peanut meal was 54.08%. In addition, protein and amino acid contents in the peanut meal were measured, and it was found that fermentation with Lactobacillus paracasei YS-816 enhanced the protein and amino acid contents by 1.73% and 1.53%, respectively. Liquid fermentation experiments were further conducted to investigate the detoxification mechanism. These revealed the adsorption capacity of Lactobacillus paracasei YS-816 suspension to be stronger than the degradation ability of the supernatant. Furthermore, the complex formed between Lactobacillus paracasei YS-816 and AFB1 maintained a high adsorption rate even after repeated elution with phosphatebuffered saline (PBS) and digestion in simulated gastric juice. Thus, Lactobacillus paracasei YS-816, screened from traditional sauerkraut, was shown to effectively detoxify AFB1 from peanut meal with good adsorption stability. This study provides a scientific basis for the application of Lactobacillus paracasei in the detoxification of aflatoxins in feed materials like peanut meal.
