定量分析不同油脂的脂肪酸组成及其与抗氧化能力和氧化稳定性之间的关系
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刘峻辰(2001-),男,硕士研究生,研究方向:化妆品配方创新及原料改性,E-mail:l15382166411@163.com 通讯作者:李丽(1980-),女,博士,教授,研究方向:中国特色植物原料研发与应用,E-mail:lili2212@163.com

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Quantitative Analysis of the Fatty Acid Compositions of Different Oils and Associations with Antioxidant Capacity and Oxidative Stability
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    摘要:

    植物油中主要组成为脂肪酸,为确定小宗植物油与一些研究较少的功效性植物油的脂肪酸组成并且研究其与抗氧化活性和氧化稳定之间的关系。该研究使用气相色谱-质谱法测定其脂肪酸组成,并通过植物油的DPPH清除能力来测试植物油的抗氧化活性以IC50值表示,以此表征其对人体健康的影响,使用油脂氧化分析测试油脂氧化诱导时间表征其氧化稳定性,并通过将植物油的脂肪酸组成与DPPH自由基清除能力和氧化稳定性进行关联性分析。结果显示:咖啡籽油的饱和脂肪酸含量最高(355.10 mg/g)、青刺果油的不饱和脂肪酸含量最高(844.84 mg/g);咖啡籽油的DPPH IC50值最低(2.30 mg/mL),氧化诱导时间最长为17.09 h。经相关性分析后植物油的氧化稳定性(P<0.05)与不饱和脂肪酸含量呈显著的线性相关,不饱和脂肪酸含量越低,氧化稳定性越好。该研究为功能性食用植物油的筛选提供了参考数据。

    Abstract:

    Fatty acids are the main constituents of vegetable oils. To determine the fatty acid compositions of small trade vegetable oils and some less well studied beneficial vegetable oils, and investigate their relationships with antioxidant activity and oxidative stability, gas chromatography-mass spectrometry was performed to characterize the associated fatty acid profiles. The antioxidant activity of vegetable oils, based on their DPPH-scavenging capacity (expressed as IC50 values), was used to assess their impact on human health, and their oxidative stability was characterized by performing lipid oxidation analysis to determine the oxidative induction time of fats and oils. In addition, correlation analyses were performed to examine associations between the fatty acid composition of the oils and DPPH-scavenging capacity and oxidative stability. The results revealed that among the assessed oils, coffee seed oil has the highest saturated fatty acid content (355.10 mg/g), whereas Garddenia jaminoides oil has the highest unsaturated fatty acid content (844.84 mg/g). Coffee seed oil was also found have the lowest DPPH IC50 value (2.30 mg/mL) and the longest oxidation induction time (17.09 h). Correlation analysis revealed a significant linear relationship (P<0.05) between oxidative stability and unsaturated fatty acid content, with lower contents tending to be associated with better oxidative stability. The findings of this study provide reference data for the screening of functional edible vegetable oils.

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刘峻辰,孙晓曼,张慧荣,邵浩凡,凌霄,李丽.定量分析不同油脂的脂肪酸组成及其与抗氧化能力和氧化稳定性之间的关系[J].现代食品科技,2025,41(4):305-315.

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  • 收稿日期:2024-02-04
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  • 在线发布日期: 2025-05-08
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