强化钙铁锌硒酒糟的制备及其体外Caco-2细胞转运吸收特性的分析

Fortified Distillers’ Grains with Calcium, Iron, Zinc, and Selenium: Prep aration and in Vitro Absorption in Caco-2 Cells

摘要:

为了实现生物强化食品有机态矿物质，该研究对大米发酵酿酒工艺进行了矿物质（钙、铁、锌、硒）强化，同时采用人结肠腺癌细胞（Caco-2）模型对富矿酒糟中钙、铁、锌、硒的转运效果进行了研究。结果表明，在大米基料中钙、铁、锌、硒添加量分别为4 000、600、1 000、30 mg/kg时，获得的富矿酒糟中有机态钙、铁、锌、硒含量分别为169.21、19.28、9.34、11.07 mg/kg；细胞实验结果表明，富矿酒糟钙、铁、锌元素的转运率均显著高于无机矿物质对照组（P<0.05），钙转运率为50.30%、铁转运率60.89%、锌转运率为35.07%，硒的转运率为10.30%。分别较空白组提升3.00倍、12.63倍、11.77倍、2.49倍。综上实验结果，通过大米发酵酿酒生物强化矿物质的酒糟具有更高的钙、铁、锌、硒含量及转运吸收作用，这为优质矿物质强化食品和保健品的制备提供了新策略和理论基础。

Abstract:

Mineral fortification with calcium, iron, zinc, and selenium was performed during the rice fermentation brewing process to obtain mineral-rich distillers’ grains. Additionally, the transport of these minerals in the mineral-rich lees was studied using the human Caco-2 colon cell line. The mineral-rich distillers’ grains obtained after addition of 4 000, 600, 1 000, and 30 mg/kg (mass fraction), respectively, of calcium, iron, zinc, and selenium to the rice base contained 169.21, 19.28, 9.34, and 11.07 mg/kg of the respective mineral. The cell experiment results showed that the transport rates of calcium, iron, and zinc in the mineral-rich distillers’ grains were significantly higher than those in the inorganic mineral control group grains (50.30%, 60.89%, and 35.07%, respectively; P<0.05). The transport rates of calcium, iron, zinc, and selenium were significantly higher than the inorganic control group, with an increase of 3.00, 12.63, 11.77, and 2.49 times, respectively. Together, these results indicate that mineral-fortified distillers' grains from rice fermentation possess enhanced transport and absorption properties, offering a promising strategy for developing high-quality mineral-fortified foods and dietary supplements.