低浓度敏感型猪血浆蛋白-黄原胶酸诱导冷凝胶表征
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马转转(1998-),女,硕士研究生在读,研究方向:蛋白质资源综合利用,E-mail:18735835096@163.com 通讯作者:袁江兰(1970-),女,博士,教授,研究方向:蛋白质资源综合利用,E-mail:jlyuan1229@163.com

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工业发酵省部共建协同创新中心开放基金项目


Characterization of Low Concentration-sensitive Porcine Plasma Protein-Xanthan Gum and Acid-induced Cold-set Gel
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    摘要:

    该文以葡萄糖酸内酯(GDL)为酸化剂调节猪血浆蛋白(PPP)-黄原胶(XG)复合溶液的pH值至3.5,从而形成稳定的PPP-XG复合凝胶。质构、流变、电镜分析结果表明低含量的PPP和XG即可形成典型凝胶,具有致密的网络结构。电位和相互作用力证明了PPP和XG之间发生了非共价相互作用,静电和氢键为主要驱动力。在一定质量分数的多糖条件下,蛋白质与多糖比为1:1时,凝胶具有最大的硬度。而在其他条件下,凝胶硬度出现下降趋势。XG对凝胶硬度有促进作用。当XG质量分数为0.3%时,混合溶液具有合适的粘度和硬度,PPP质量分数为0.3%时具有最大的硬度为250.02 g,而此时持水力最低为53.96%,协同作用值最高为9.77%,储能模量和分子间作用力最强,凝胶结构致密。而溶胀性能测定表明该复合凝胶在pH值7.4条件下的吸水能力明显优于pH值2.0(P<0.05)。研究表明该复合凝胶可改善食品的质地特性,并可作为潜在热敏性成分的递送载体,为食品加工提供参考。

    Abstract:

    The pH of porcine plasma protein (PPP)-xanthan gum (XG) composite solution was adjusted to 3.5 using gluconolactone (GDL) as an acidifying agent, resulting in the formation of stable PPP-XG composite gels. The results of textural, rheological, and electron microscopic analyses showed that even low concentrations of PPP and XG could form typical gels with dense network structures. The Zeta potential and interaction force studies demonstrated that non-covalent interactions occurred between PPP and XG, with electrostatic and hydrogen bonding as the primary driving force. Under specific mass fraction of polysaccharide, the gel had the maximum hardness at a protein-to-polysaccharide ratio of 1:1. However, under other conditions, the gel hardness showed a decreasing trend. XG had a promoting effect on the gel hardness. When the mass fraction of XG was 0.3%, the mixed solution had appropriate viscosity and hardness. The gel with a PPP mass fraction of 0.3% had the maximum hardness of 250.02 g, with the lowest water retention capacity of 53.96%, highest synergistic value of 9.77%, and strongest energy storage modulus and intermolecular forces, resulting in a dense gel structure. The swelling property measurement showed that the water absorption capacity of the composite gel at a pH value of 7.4 was significantly higher than that at a pH of 2.0 (P<0.05). This study indicated that the composite gel could improve the texture characteristics of food products and serve as a delivery vehicle for thermosensitive ingredients, providing valuable insights for food processing.

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马转转,康旭,袁江兰.低浓度敏感型猪血浆蛋白-黄原胶酸诱导冷凝胶表征[J].现代食品科技,2025,41(4):257-265.

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  • 收稿日期:2024-03-02
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  • 在线发布日期: 2025-05-08
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