Abstract:In order to realize the efficient utilization of Grifola frondosa Polysaccharide (GFP), a novel Deep Eutectic Solvent (DES) was used to extract GFP. The extraction conditions were optimized and the antioxidant activity of the GFP was investigated. Grifola frondosa was used as the raw material, DES-2 (choline chloride-ethylene glycol) was found as the optimal polysaccharide extraction solvent among the eight DESs after screening. On the basis of single factor experiments, the orthogonal experimental design was used to optimize the extraction conditions of GFP. The optimal extraction condition was determined as follows: DES-2 water content, 10%; DES-2 molar ratio, 1:1; liquid- to-material ratio, 80 mL/g; extraction temperature, 60 ℃; extraction time, 40 min. Under such conditions, the extraction yield of GFP was 30.25%, which was 2.23 times that of the traditional hot water extraction method. The results of antioxidant studies indicated that the polysaccharide extracted by hot water (W-GFP) had EC50 values of 0.11, 0.45 and 0.26 mg/mL in the DPPH, ABTS and •OH radical scavenging tests, respectively, whilst the G. Frondosa polysaccharides extracted by eutectic solvents (D-GFPs) had EC50 values of 0.11, 0.56 and 0.23 mg/mL, respectively . Therefore, this study showed that extraction of G. Frondosa by DES had the advantages of being green and having high yield, and the obtained GFPs exhibited relatively strong antioxidant activities, which provides a theoretical basis for the development and utilization of GFP functional foods.
