活性海星肽的分离纯化及其对人乳腺癌细胞的作用

Isolation and Purification of Active Starfish Peptides and Their Effects on Human Breast Cancer Cells

摘要:

为了从海星中分离纯化出活性肽并探究其对人乳腺癌细胞MCF-7的作用，采用仿生酶解法制备了海星肽粗品。通过阴离子交换层析法和凝胶柱层析法进行了分离纯化，得到了两组活性海星肽，利用MTT法比较了这两组活性海星肽对MCF-7人乳腺癌细胞的抑制作用的强弱；海星肽粗品经阴离子交换层析法分离获得A1、A2、A3、A4、A5五种组分后，再通过凝胶柱层析法分离获得B1、B2、B3三种组分，利用MCF-7细胞作为筛选模型作抗肿瘤活性鉴定。通过阴离子交换层析法获得的组分A2质量浓度为600 μg/mL时可比较有效地抑制MCF-7细胞生长，抑制率为21.65%；通过凝胶柱层析法分离获得的组分B1、B2、B3均具有明显的抗肿瘤活性，其中B1质量浓度为200 μg/mL时能有效抑制MCF-7细胞生长，抑制率为14.65%；B2、B3质量浓度为300 μg/mL时能有效抑制MCF-7细胞生长，抑制率分别为21.23%、24.68%。研究表明通过阴离子交换层析和凝胶柱层析从海星中分离纯化出的活性肽组分对人乳腺癌细胞MCF-7呈现出明显的抑制作用，这为进一步研究海星肽活性组分的抗肿瘤性能提供了依据。

Abstract:

Raw starfish peptides were prepared by biomimetic enzymatic hydrolysis to isolate and purify active peptides from starfish and explore their effects on human breast cancer cells MCF-7. Two groups of active starfish peptides were separated and purified by anion exchange chromatography and gel column chromatography. The MTT method was used to compare the inhibitory effect of these two groups of active starfish peptides on MCF-7 human breast cancer cells. The crude starfish peptide was separated by anion exchange chromatography to obtain five components, A1, A2, A3, A4 and A5, which were then separated by gel column chromatography to obtain three components, B1, B2 and B3. MCF-7 cells were used as a screening model for evaluating the antitumor activity. The A2 component obtained by anion exchange chromatography inhibits the growth of MCF-7 cells by 21.65% at an A2 concentration of 600 μg/mL. The B1, B2 and B3 components obtained by separation using gel column chromatography showed significant anti-tumor acitivites. B1 at 200 μg/mL significantly inhibited the growth of MCF-7 cells, with an inhibition rate of 14.65%. B2 and B3 at concentrations of 300 μg/mL could effectively inhibit the growth of MCF-7 cells, with inhibition rates of 21.23% and 24.68%, respectively. The results showed that the active peptide components isolated and purified from starfish by anion exchange chromatography and gel column chromatography exhibited significant inhibitory effects on human breast cancer cells MCF-7, which provides a basis for further research on the anti-tumor properties of starfish peptides’ active components.