环介导等温扩增技术在食品安全领域的应用进展

Progress in Application of Loop-mediated IsothermalAmplification in Food Safety Inspection

摘要:

食品安全不仅关乎人民身体健康，同时影响地区经济发展。高效、便捷的检测方法是实现风险评估和安全监管的重要手段。传统的检测技术操作繁琐、耗时颇长，难以满足市场上快速检测的需求。环介导等温扩增技术具有速度快、灵敏度高、特异性强和成本低廉等优点，显现出巨大的商业应用潜力。该文综述环介导等温扩增技术的原理、分类、产物检测方法，并重点分析基于颜色变化的比色法在环介导等温扩增技术中的应用，结合近5年来该技术在食品安全检测领域的应用进展，以期为开发快速、现场、低廉的食品安全检测方法提供参考。

Abstract:

Food safety issues impact individual physical health and regional economic development. Efficient and convenient detection methods are key to achieving relevant risk assessment and safety regulation. Traditional detection techniques are cumbersome and time-consuming, and fail to meet the market demand for rapid detection. Loop-mediated isothermal amplification (LAMP) is a promising candidate method for commercial applications in food safety inspection owing to its high speed, high sensitivity, strong specificity, and low cost. This study reviews the principle, classification, and product detection methods of LAMP, with a focus on analyzing the application of colorimetry in LAMP, and summarizes progress made in food safety inspection in the past five years, aiming to provide a reference for developing fast, on-site, lowcost methods for food safety inspection.