甲鱼肽的分离纯化及对小鼠氧化损伤的改善作用

Separation and Purification of soft-shelled turtle peptides and its ameliorative effect against oxidative damage in mice

摘要:

为了探究甲鱼肽对小鼠氧化损伤的改善作用，该研究首先以DPPH自由基清除率为评价指标，通过离子交换树脂法优化甲鱼肽（Soft-Shelled Turtle Peptides，STP）的分离纯化工艺获得高活性甲鱼抗氧化肽。随后建立D-半乳糖（D-galactose，D-gal）诱导的小鼠氧化损伤模型，采用试剂盒检测小鼠各组织的超氧化物歧化酶（Superoxide Dismutase，SOD）、谷胱甘肽过氧化物酶（Glutathione Peroxidase，GSH-Px）和丙二醛（Malondialdehyde，MDA）含量。结果表明，凝胶型阳离子交换树脂（Gel Cation Ion Exchange Resin，GC）分离纯化甲鱼抗氧化肽效果最佳，其静态吸附pH值为1.0和解吸附溶液乙醇体积分数为50%时，DPPH自由基清除率提高了34.42%（P<0.001）。与模型组相比，GC纯化后甲鱼抗氧化肽（Purified of STP with GC，GC-STP）组肝脏和胸腺组织的SOD活性显著增加62.28%（P<0.05）和59.18%（P<0.01）；肝脏、脾脏和胸腺组织的GSH-Px活性显著增加32.52%（P<0.05）、7.50%（P<0.05）和178.96%（P<0.01）；肝脏组织的MDA含量显著降低28.28%（P<0.05）。综上所述，GP-STP改善D-gal诱导小鼠肝脏组织氧化损伤。该研究可为GP-STP改善组织氧化氧化损伤提供理论基础。

Abstract:

The antioxidant peptides from the soft-shelled turtle was isolated and purified, and the ameliorative effect of purified peptides on the oxidative damage in mice was evaluated. The DPPH radical scavenging rate was used as the evaluation indicator for optimizing the separation and purification process of ion exchange resin method to obtain the high activity antioxidant peptides from soft-shelled turtle (STP). Subsequently, D-galactose (D-gal) -induced oxidative damage model was established in mice, and ELISA kits was used to measure superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity, and malondialdehyde (MDA). The results showed that gel cation ion exchange resin (GC) was the best for separation and purification of antioxidant peptides. Under the conditions of 1.0 of pH value and 50% of ethanol volume fraction, the DPPH radical scavenging rate was increased by 34.42% (P<0.001) when compared with STP. Compared to the model group, the SOD activity in liver and thymus tissues of purified of turtle antioxidant peptides with GC (GC -STP) group was significantly increased by 62.28% (P<0.05) and 59.18% (P<0.01); The GSH-Px activity in liver, spleen and thymus tissues was significantly increased by 32.52% (P<0.05), 7.50% (P<0.05) and 178.96% (P<0.01); The malondialdehyde (MDA) content in liver was significantly decreased by 28.28% (P<0.05). Therefore, GP-STP could effectively ameliorate D-gal induced oxidative damage of live tissue in mice. The findings of this study serve as the theoretical foundation of GP-STP to improve oxidative damage.