为了实现对葡萄糖的快速、高灵敏检测，该研究采用二维碳基纳米材料功能化边缘改性石墨烯（FG）作为导电载体，葡萄糖氧化酶（GOx）作为生物识别分子，构建了检测葡萄糖的高性能电化学生物传感器（GCE/FG/GOx/CS）。实验采用循环伏安法（CV）和时间-电流法（i-t），探讨了GCE/FG/GOx/CS电极的电化学传感性能。结果表明，该传感电极具有良好的电传感性能，对葡萄糖具有较高的检测灵敏度（44.30 μA·mmol·L-1cm-2）和线性检测范围（0.05 mmol/L~1.40 mmol/L），检测限低至2.5 μmol/L。此外，该电极还具有良好的选择性、重现性（n=7，相对标准偏差RSD=2.05%）、循环稳定性和贮存稳定性（一周后响应电流保持率为97.09%）。用于实际饮料样品中葡萄糖含量的测定时，其加标回收率为98.5%~104.4%。该方法操作简便、选择性好、灵敏度高，为实现食品中葡萄糖含量的快速检测提供了理论依据。

In order to achieve rapid and highly sensitive detection of glucose, this study used two-dimensional carbon-based nanomaterials functionalized edge-modified graphene (FG) as a conductive carrier and glucose oxidase (GOx) as a biorecognition molecule to construct a high-performance electrochemical biosensor for glucose detection (GCE/FG/GOx/CS). The electrochemical sensing performance of GCE/FG/GOx/CS electrodes was investigated using cyclic voltammetry (CV) and time-current (i-t) methods. The results showed that the sensing electrode had good electrosensing performance, high sensitivity (44.30 μA·mmol·L-1cm-2) and linear detection range (0.05 mmol/L~1.40 mmol/L) for glucose, and the detection limit was as low as 2.5 μmol/L. In addition, the electrode showed good selectivity, reproducibility (n=7, relative standard deviation RSD=2.05%), cycling stability and storage stability (97.09% retention of response current after one week). The recoveries were 98.5%~104.4% for the determination of glucose in real beverage samples. The method is simple, selective and sensitive, and provides a theoretical basis for realizing the rapid detection of glucose content in food.

宁夏重点研发计划（NO.2022BDE02004）;研究生科技创新项目（CXXM2024-29）;珠海市产学研合作项目（NO.2220004002701）;宁夏大学贺兰山学者项目和宁夏青年人才支持计划（2020）